Assistant Professor
313-577-1993
Fax: 313-577-0798
d.nie@wayne.edu
431 Chemistry Bldg.,Detroit, Michigan 48202

Research Interests

Angiogenesis, cellular signaling in carcinoma radiation response, G-protein coupled receptors in tumor progression, Bone metastasis

Curriculum Vitae

Nie, D., Genge, B.R., Wu, L.N.Y., and Wuthier, R.E. 1995. Defects in formation of functional matrix vesicles by growth plate chrondrocytes in avian tibial dyschondroplasia: Evidence of defective tissue vascularization. J Bone Miner Res 10:1625-1634.

Wu, L.N.Y., Ishikawa, Y., Nie, D., Genge, B.R., and Wuthier, R.E. 1997. Retinoic acid stimulates matrix calcification and initiates type I collagen synthesis in primary cultures of avain growth plate chondrocytes. J Cell Biochem 65:209-230.

Nie, D., Ishikawa, Y., Yoshimori, T., Wuthier, R.E., and Wu, L.N.Y. 1998. Retinoic acid treatment elevates matrix metalloproteinase-2 protein and mRNA levels in avian growth plate chondrocyte cultures. J Cell Biochem 68:90-99.

Sauer, G.R., Nie, D., Wu, L.N.Y., and Wuthier, R.E. 1998. Induction and characterization of metallothionein in chicken epiphyseal growth plate cartilage chondrocytes. J. Cell. Biochem. 68: 110 - 120.

Nie, D., Ishikawa, Y., Guo, Y., Wu, L.N., Genge, B.R., Wuthier, R.E., and Sauer, G.R. 1998. Inhibition of terminal differentiation and matrix calcification in cultured avian growth plate chondrocytes by Rous sarcoma virus transformation. J. Cell. Biochem. 69: 453 -462.

Nie, D., Hillman, G.G., Geddes, T., Tang, K., Pierson, C., Grignon, D.J., and Honn, K.V. 1998. Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth. Cancer Res 58: 4047 - 4051.

Tang, K., Nie, D., Cai, Y., and Honn, K.V. 1999. The beta4 integrin subunit rescuesA431 ccells from apoptosis through a PI3K/Akt kinase signaling pathway. Biochem. Biophys. Res. Commun. 264: 127 - 132.

Nie, D., Lamberti, M., Zacharek, A, Li, L., Szekeres, K., Tang, K., Chen, Y., and Honn, K.V. 2000. Thromboxane A(2) regulation of endothelial cell migration, angiogenesis, and tumor metastasis. Biochem. Biophys. Res. Commun. 267: 245- 251.

Tang, K., Finley, R.L. Jr, Nie, D., and Honn, K.V. 2000. Identification of 12-lipoxygenase interaction with cellular proteins by yeast two-hybrid screening. Biochemistry 39: 3185 - 3191.

Nie, D., Tang, K., Diglio, C., and Honn, K.V. 2000. Eicosanoid regulation of angiogenesis: role of endothelial arachidonate 12-lipoxygenase. Blood 95: 2304 - 2311.

Timar, J., Raso, E., Dome, B., Li, L., Grignon, D., Nie, D., Honn, K.V., and Hagmann, W. 2000. Expression, subcellular localization and putative function of platele-type 12-lipoxygenase in human prostate cancer cell lines of different metastatic potential. Int. J. Cancer 87: 37 - 43.

Szekeres, C.K., Trikha, M., Nie, D., and Honn, K.V. 2000. Eicosanoid 12(S)-HETE activates phosphatidylinostol 3-kinase. Biochem. Biophys. Res. Commun. 275: 690 - 695. 

Nie, D., K. Tang, K. Szekeres, M. Trikha, and K. V. Honn. Role of eicosanoids in tumor growth and metastasis. Advances in Eicosanoid Research (C.S. Serhan, ed.). Springer, Berlin. pp. 201 - 217, 2000.

Nie, D., M. Che, D. Grignon, K. Tang, and K. V. Honn. Role of eicosanoids in prostate cancer progression. Cancer and Metastasis Review 20: 195 - 206, 2001.

Nie, D. and K.V. Honn. Cyclooxygenase, lipoxygenase, and tumor angiogenesis. Cell Mol Life Sci. 59: 799 - 807, 2002.

Wu LN, Lu M, Genge BR, Guo GY, Nie D, Wuthier RE. Discovery of Sonic hedgehog expression in postnatal growth plate chondrocytes: Differential regulation of Sonic and Indian hedgehog by retinoic acid. J Cell Biochem 87: 173 - 187, 2002.

Nie D, J Nemeth, Y Qiao, L Li, K Tang, GG Hillman, ML Cher, DJ Grignon, and KV Honn. Increased Metastatic Potential by Overexpression of Arachidonate 12-Lipoxygenase in Human Prostate Cancer Cells. Clinical and Experimental Metastasis 20: 657 - 663, 2003.

Kandouz M, Nie D, Pidgeon GP, Krishnamoorthy S, Maddipati KR, and Honn KV. Platelet-type 12-Lipoxygenase Activates NF-kappa B in Prostate Cancer Cells. Prostaglandins and other Lipid Mediators 71: 189 - 204, 2003.

Nie D, Che M, Zacharek, A; Qiao, Y; Cai Y; Tang, K; Lamberti, M; Grignon, D and Honn, KV. Thromboxane Synthase Expression in Prostate Cancer: Involvement in Perineural Invasion. American Journal of Pathology 164: 429 - 439, 2004

Nie D, Y Chen, Y Qiao, A Zacharek, K Tang, J Milanini, G Pages, D Grignon, and KV Honn. Arachidonate 12-Lipoxygenase regulates the expression of vascular endothelial growth factor in a PI3 kinase dependent pathway. Submission to FASEB Journal.

Nie D; Qiao Y; Zacharek, A and Honn, KV. Regulation of prostate cancer metastatic phenotypes by thromboxane A2 through small GTPase Rho. In submission to the Journal of Biological Chemistry.

PATENTS:
Wuthier, R.E., Nie, D. Angiogenesis inhibitor for the treatment of cancer. U.S. Patent No. 5,932,611. August 3, 1999.

Wuthier, R. E., Nie, D. Angiogenesis Inhibitor for the treatment of endothelial cell proliferation. U.S. Patent No. 6,225,340. May 1, 2001.

Wuthier, R.E. and Nie, D. Use of FC101 as an angiogenesis inhibitor for the treatment of cancer and other angiogenic diseases. European Patent EP1049463B1. May 14, 2003.